28 décembre 2012

Associations of BAX with chemical compounds

Coexpression of BAD and NOXA killed wild-type but not Bax, Bak doubly deficient cells or Puma deficient cells with Bim knockdown, indicating that activator BH3-only molecules function downstream of inactivator BH3-only molecules to activate BAX-BAK. These results suggest that glutathione-dependent BAX activation in cells with normal CFTR represents an early step in oxidative stress-induced apoptosis of these cells. The expression of BCL-2 and BAX, which would occur downstream from p53, was not changed by irradiation and Trolox... [Lire la suite]
Posté par tnfalpha à 03:06 - Commentaires [0] - Permalien [#]

28 décembre 2012

BAX treatment

Interestingly, LDFRT treatment in both cell lines with or without Paclitaxel down-regulated nuclear factor kappa B activity and BCL-2 protein expression and simultaneously up-regulated BAX protein. Recent genetic studies with fibroblasts derived from mutant mouse embryos indicate that a class of the BCL-2 family proapoptotic proteins (designated BH-123 or multidomain proteins) such as BAX and BAK constitutes an essential component of the core apoptosis machinery in animal cells. No germ line BAX mutations were found.
Posté par tnfalpha à 03:05 - Commentaires [0] - Permalien [#]
27 décembre 2012

High impact information on BAX

Moreover, BCL-XL, BCL-2, and BAX can form ion-conductive pores in artificial membranes [5]. The percentage of myocytes labeled with BCL2 (which protects cells against apoptosis) was 1.8 times as high in the hearts of patients with cardiac failure as in the normal hearts, whereas labeling with BAX (which promotes apoptosis) remained constant. Bax (Bcl2-associated X protein) is an apoptosis-inducing protein that participates in cell death during normal development and in various diseases.
Posté par tnfalpha à 02:31 - Commentaires [0] - Permalien [#]
27 décembre 2012

Elevated BAX

Surprisingly, we observed elevated BAX levels in patients with thyroid carcinomas compared with patients with adenomas (unpaired t-test: p<0.05) or with goitres (p<0.02). We conclude that BAX expression may represent a prognostic indicator for patients with ovarian cancer and that the combined evaluation of BAX and BCL-2 may provide additional prognostic significance.
Posté par tnfalpha à 02:31 - Commentaires [0] - Permalien [#]
27 décembre 2012

Disease relevance of BAX

Loss of BAX also renders colorectal cancer cells resistant to TRAIL/Apo2L-mediated radiosensitization. These same v-BCL-2 proteins cooperate with loss of retinoblastoma protein and p53 tumor suppressor function, by inactivating the BAX and BAK apoptotic pathway to promote epithelial solid tumor growth and resistance to chemotherapy. This is in clear contrast to other carcinomas where BAX is frequently inactivated which correlates to a poor prognosis . There were no significant differences of the BAX levels between goitres or the... [Lire la suite]
Posté par tnfalpha à 02:31 - Commentaires [0] - Permalien [#]
26 décembre 2012

mRNA of BDNF

In situ hybridizations for BDNF and trkB mRNA revealed that virtually all CSNs express trkB, whereas only half of them express BDNF. As revealed with 5-HT immunocytochemistry, BDNF infusions into the neocortex of intact (non-PCA-lesioned) rats caused a substantial increase in 5-HT axon density in a 3 mm diameter region surrounding the cannula tip.
Posté par tnfalpha à 01:55 - Commentaires [0] - Permalien [#]

26 décembre 2012

Analytical, diagnostic and therapeutic context of Bdnf

The increase in BDNF mRNA was mediated by a selective activation of the BDNF exon IV promoter and adrenalectomy attenuated this increase by 50%. In bioassays, BDNF and NT-3, but not NGF, at physiological concentrations induced neurite outgrowth from the statoacoustic ganglion explants.
Posté par tnfalpha à 01:55 - Commentaires [0] - Permalien [#]
26 décembre 2012

Immunoreactivity of BDNF

The distribution of BDNF-induced neuropeptide Y immunoreactivity is similar to the pattern observed in animals submitted to hippocampal kindling, with the exception of mossy fibres which only become immunoreactive following seizure activity. We found that single or repeated immobilization markedly reduced brain-derived neurotrophic factor (BDNF) mRNA levels in the dentate gyrus and hippocampus.
Posté par tnfalpha à 01:55 - Commentaires [0] - Permalien [#]
25 décembre 2012

BDNF in neuron

Although hippocampal neurons do not express typical LDCV proteins, transfected chromogranins A, B, and brain-derived neurotrophic factor (BDNF) colocalize with VMAT2.
Posté par tnfalpha à 02:09 - Commentaires [0] - Permalien [#]
25 décembre 2012

Expression of BDNF

These factors may act as the eventual repressors for BDNF expression via competition and heterodimerization with phosphorylated CREB, a transcription factor important for BDNF expression. Shp-2 specifically regulates several tyrosine-phosphorylated proteins in brain-derived neurotrophic factor signaling in cultured cerebral cortical neurons.
Posté par tnfalpha à 02:08 - Commentaires [0] - Permalien [#]