30 décembre 2012

Psychiatry related information on BCHE

In Alzheimer's disease, BCHE may have thus acquired an inverse role to that of AChE by adopting imperfect amphipathic characteristics of its C terminus. Our results point to a possible association between an abnormal lipid metabolism and the BChE activity and might have implications as regards the pathomechanism of both Alzheimer's and vascular dementias and the cholinesterase inhibitor therapy of dementing disorders.
Posté par tnfalpha à 02:32 - Commentaires [0] - Permalien [#]

30 décembre 2012

Restriction analysis of BCHE

PvuII restriction analysis and DNA blot hybridization of the amplified ACHE and BCHE sequences demonstrated apparent aberrations in both genes, suggesting that malfunctioning of modified, partially amplified cholinesterase genes may be involved in the etiology of thrombocytopenia associated with SLE. At the clinical level, abnormal expression of both ACHE and BCHE and the in vivo amplification of the ACHE and BCHE genes has been variously associated with abnormal megakaryocytopoiesis, leukemias and brain and ovarian tumors.
Posté par tnfalpha à 02:32 - Commentaires [0] - Permalien [#]
29 décembre 2012

Entrez Gene summary for BAX

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAX forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is... [Lire la suite]
Posté par tnfalpha à 01:30 - Commentaires [0] - Permalien [#]
29 décembre 2012

BAX in immunoblotting

Significantly highernumbers of BAX positive hepatocytes were observed in HBsAg positive patients when compared to control groups. Immunoblotting revealed that BAX transfectants expressed a mean of 10-fold increased levels of BAX compared with neo-transfected control clones, with similar levels of BCL-2 and BCL-xL.
Posté par tnfalpha à 01:29 - Commentaires [0] - Permalien [#]
29 décembre 2012

Analytical, diagnostic and therapeutic context of BAX

In 47 patients with thyroid tumours (14 follicular and 3 papillary carcinomas, 14 adenomas and 16 goitres), the DNA was screened for mutations of BAX (exon 1-6) and p53 (exon 5-8) by single-strand conformation polymorphism polymerase chain reaction (SSCP-PCR). The levels of p53, BAX, BCLl-2 and p21 mRNA expression were assessed by real time RT-QPCR and the presence of respective proteins in the vessel wall was confirmed by immunohistochemistry.
Posté par tnfalpha à 01:29 - Commentaires [0] - Permalien [#]
28 décembre 2012

Cytotoxicity of BAX

The cytotoxicity of paclitaxel, vincristine, and doxorubicin was significantly enhanced in BAX transfectants compared with control clones, whereas the cytotoxicity profile of carboplatin, etoposide, and hydroxyurea was unchanged. The inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (zVAD-fmk) successfully blocked this protease activity and prevented FAS-induced death but not BAX-induced death.
Posté par tnfalpha à 03:06 - Commentaires [0] - Permalien [#]

28 décembre 2012

Associations of BAX with chemical compounds

Coexpression of BAD and NOXA killed wild-type but not Bax, Bak doubly deficient cells or Puma deficient cells with Bim knockdown, indicating that activator BH3-only molecules function downstream of inactivator BH3-only molecules to activate BAX-BAK. These results suggest that glutathione-dependent BAX activation in cells with normal CFTR represents an early step in oxidative stress-induced apoptosis of these cells. The expression of BCL-2 and BAX, which would occur downstream from p53, was not changed by irradiation and Trolox... [Lire la suite]
Posté par tnfalpha à 03:06 - Commentaires [0] - Permalien [#]
28 décembre 2012

BAX treatment

Interestingly, LDFRT treatment in both cell lines with or without Paclitaxel down-regulated nuclear factor kappa B activity and BCL-2 protein expression and simultaneously up-regulated BAX protein. Recent genetic studies with fibroblasts derived from mutant mouse embryos indicate that a class of the BCL-2 family proapoptotic proteins (designated BH-123 or multidomain proteins) such as BAX and BAK constitutes an essential component of the core apoptosis machinery in animal cells. No germ line BAX mutations were found.
Posté par tnfalpha à 03:05 - Commentaires [0] - Permalien [#]
27 décembre 2012

High impact information on BAX

Moreover, BCL-XL, BCL-2, and BAX can form ion-conductive pores in artificial membranes [5]. The percentage of myocytes labeled with BCL2 (which protects cells against apoptosis) was 1.8 times as high in the hearts of patients with cardiac failure as in the normal hearts, whereas labeling with BAX (which promotes apoptosis) remained constant. Bax (Bcl2-associated X protein) is an apoptosis-inducing protein that participates in cell death during normal development and in various diseases.
Posté par tnfalpha à 02:31 - Commentaires [0] - Permalien [#]
27 décembre 2012

Elevated BAX

Surprisingly, we observed elevated BAX levels in patients with thyroid carcinomas compared with patients with adenomas (unpaired t-test: p<0.05) or with goitres (p<0.02). We conclude that BAX expression may represent a prognostic indicator for patients with ovarian cancer and that the combined evaluation of BAX and BCL-2 may provide additional prognostic significance.
Posté par tnfalpha à 02:31 - Commentaires [0] - Permalien [#]